HOW DO I PLACE AN ORDER?
To place your order visit our Order page to complete the online form. Xona Microfluidics, LLC accepts Visa and MasterCard only (American Express not accepted). Customers wishing to make payment by Check or Wire Transfer must provide a valid Purchase Order number on the online order form. For those customers submitting a purchase order, there is an upload feature on the online form that will allow you to upload a pdf of the purchase order. This will be submitted to us as well once you have completed the online order form. IMPORTANT: In order to receive order confirmations and shipment tracking information, please provide a contact name and valid e-mail address. You will not be able to submit your order without providing this information. Thank you. Our Hours of Operation: 10 a.m. to 5 p.m. PST Monday – Friday. Please Note: Xona Microfluidics, LLC is closed all major U.S. holidays. International Customers: If these times are inconvenient for you, please e-mail us at firstname.lastname@example.org and we will gladly arrange to take your phone call.
HOW WILL MY ORDER SHIP?
Xona Microfluidics, LLC ships orders via FedEx. If you are unable to receive FedEx deliveries, please contact us at email@example.com or let us know in the ‘Comments’ box on the online order form.
Xona Microfluidics, LLC will e-mail an order confirmation with your order details and estimated shipping date within 24 hours of receiving your order. If you do not receive an e-mail order confirmation within 24 hours please contact us at firstname.lastname@example.org.
For quantities of 8 packs or less please allow 72 hours for order processing and shipping. Orders of quantities larger than 8 packs may require more processing time than 72 hours. We will advise you on an estimated ship date for larger orders.
Our products are shipped in packs of five (5).
WHAT MATERIAL ARE THE NEURON DEVICES MADE FROM?
IS IT SAFE?
Yes, PDMS is considered biologically inert and safe for both humans and cells.
DOES PDMS INTERFERE WITH MICROSCOPY AND LIVE CELL IMAGING?
PDMS is optically transparent and suitable for most types of microscopy and cell imaging.
WHAT IS THE PURPOSE OF THE NEURON MICROFLUIDIC DEVICE?
The neuron microfluidic device allows researchers to compartmentalize neuronal cultures. The neuron device consists of two culture chambers separated by a microgroove barrier. Neurons are typically loaded on one side of the device. The size of the microgroove barrier allows axons to grow through the barrier while keeping the cell bodies (Soma) isolated from the distal chamber. Please note, however, that the microgrooves will not keep migrating cells from crossing the barrier. In addition, high density cultures may result in some cell bodies entering the microgrooves.
The neuron device also makes it possible to perform axotomy, i.e., cutting axons via vacuum aspiration in the distal chamber and observing their regrowth.
The neuron device is also small in size (total volume ~ 600 µl); thus, researchers save on the expensive reagents needed for neuronal culture.
WHAT IS FLUIDIC ISOLATION?
The neuron device consists of two chambers, 100 µm in height, connected by a microgroove barrier that is 3 µm in height. The hydrostatic pressure between the two chambers separated by the microgroove allows the researcher to fluidically isolate each chamber. This is accomplished by keeping the volumes in the wells on one side of the device higher than the other side of the device. The difference in volume creates hydrostatic pressure, thus fluidically isolating each compartment.
CAN PROTEIN LYSATE BE ISOLATED FROM THE DEVICE?
Yes. However, keep in mind that in most cases 80,000 to 200,000 cells are being loaded per device. Therefore, the amount of protein lysate obtained may or may not be suitable for your application. The amount of protein lysate from the axonal compartment will be even lower. If you seek to do a western blot with an antibody to a protein known to be low in abundance with lysate from the axonal compartment, one device may not yield enough protein. It is possible to pool lysates from several devices, however, Xona Microfluidics, LLC makes no guarantees that you will be able to detect your protein of interest.
CAN RNA BE ISOLATED FROM THE DEVICE?
Yes. However, as with protein, the low amount of cells in the device generally translates into low yields of RNA. Like for protein lysates, devices can be pooled in order to obtain enough RNA to detect low yield RNAs. Your success in isolating RNA will depend on the abundance of your target RNA.
WHAT IS PLASMA BONDING?
Plasma bonding involves using a plasma cleaner to produce reactive oxygen species on the surface of both the neuron device and glass. In addition to cleaning the glass and neuron device, the plasma cleaner also sterilizes. After treating with plasma when the neuron device comes in contact with the glass it forms a tight irreversible bond. Plasma treating also makes the surfaces hydrophilic, thereby facilitating the introduction of liquids into the device. We typically recommend the Harrick plasma cleaner.
DO I NEED A PLASMA CLEANER?
No, the neuron device can be bound to cover glass without using a plasma cleaner. It is, however, important to use clean, sterile glass. Typically we recommend using a water bath sonicator to clean the glass, followed by an ethanol bath to sterilize them. After removing from the ethanol bath, rinse the slides three times with sterile dH2O in the glass staining dish, preferably in a bio safety cabinet to help maintain sterility. Allow the slides to dry in a bio safety cabinet for several hours, preferably overnight.
Also, the neuron devices will need to be sterilized prior to use. This can be done by rinsing the devices with 70% Ethanol and allowing them to dry completely in a biosafety cabinet. The devices can also be sterilized by autoclaving. However, one needs to take care in choosing what type of container in which to autoclave the devices. Some plastic containers and bags can impart toxins into the device which will then kill the cells.
XONA takes pride in offering comprehensive customer support.
Mon – Fri
9 AM to 5 PM PST
Xona Microfluidics is Closed all U.S. Holidays
International Customers please contact Xona through the website or email Xona using info “at” xona.us if you would like to arrange a time to speak by phone.