Neuroinflammation plays an important role in the pathogenesis of neurodegenerative diseases, including Alzheimer’s disease (AD) and ALS. Microglial activation, indicative of neuroinflammation, is present in patients with AD. One of Xona’s goals is to model critical aspects AD pathology in vitro to create simple yet robust models for therapeutics testing. Our methods rely on microfluidic compartmentalization to improve manipulations and access to cells to improve testing accuracy and throughput.
In a demonstration of our capabilities, we cultured human iPSC-derived eGFP-labeled cortical glutamatergic neurons (BrainXell) together with human iPSC-derived microglia (BrainXell) restricted to the axonal compartment using our XC450 XonaChips®. We activated microglia using lipopolysaccharide (LPS), a known inflammatory molecule. Using these human-derived cells, we recorded robust microglial activation (see figure). We found additional evidence of AD-like pathology in the somatodendritic compartment (not shown here). If you would are interested in learning more about this experiment and our results showing AD-like pathology, please contact us at info@xona.us.
At Xona we are happy to share our data and results. We also provide contract services to facilitate your research and progress toward development of effective therapeutics. We look forward to learning about your research.
ABOUT XONA
Xona Microfluidics is a US-based company that makes compartmentalized microfluidic chambers for culturing neurons, and creates co-culture and innervation models in vitro. Xona’s technology is also used to model multi-cellular environments for organ-on-a-chip or microphysiological system applications.
Our patented compartmentalized chip technology directs the growth of axons from a somatodendritic compartment to an isolated axonal compartment allowing distinct microenvironments within each compartment to be established. Xona sells compartmentalized microfluidic chambers in two formats: Preassembled XonaChips® and original Silicone (PDMS) Devices. Over 1,000 publications cite our compartmentalized microfluidic devices and chips. Many of these publications can be found on our publications page.
Xona provides contract research services in addition to product sales, using our microfluidic platforms to develop scalable neuron-based assays.
CONTACT US
To learn more about products and research services provided by Xona, please contact us at info@xona.us.
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